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The aim of this study was to formulate and evaluate a lipid-based buccal delivery system for amantadine hydrochloride for the treatment of Parkinson's disease. Amantadine hydrochloride was loaded into preformed liposomes prepared by hydration method. The lyophilized formulations were characterized for particle size, morphology, thermodynamic parameters, lipophilicity and entrapment efficiency. In vitro release studies were carried out in pH 7.4 using modified dialysis bag. Additionally, the effect of lipid type on release of drug from the formulations was assessed using alkyl, diacetyl glyceryl monostearate, distearoyl phosphatidylcholine and a mixture of distearoyl phosphatidylcholine and stearoyl phosphatidylcholine. The formulations were evaluated for various in vivo pharmacokinetic parameters such as bioavailability and duration of drug action in rat brain. The formulation had a mean diameter of 121.47+/-1.49 nm and entrapment efficiency of 60.03+/-0.02%. In vitro release studies revealed a pH-dependent release of amantadine hydrochloride. The lipid-based formulation produced a significantly higher brain level of drug than the unencapsulated amantadine hydrochloride. The ratio of brain to plasma concentration was 5.15 at 2 hours indicating that the system provided prolonged delivery of the drug in the brain. Lipid-based amantadine hydro 0b46394aab
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