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Finally, gnotobiology has been claimed as the strongest evidence against the existence of microbiomes in the fetal environment because of the ability to derive germfree animals via C-sections and subsequently raise the offspring in a sterile environment (66). This fact has to be taken in account, although on the other hand, it may be challenging to transfer results obtained in a germfree system to those derived from a conventional host (142). 1e1e36bf2d